Guidance of zoospores by potassium gradient sensing mediates aggregation.
Identifieur interne : 000486 ( Main/Exploration ); précédent : 000485; suivant : 000487Guidance of zoospores by potassium gradient sensing mediates aggregation.
Auteurs : Eric Galiana [France] ; Celine Cohen [France] ; Philippe Thomen [France] ; Catherine Etienne [France] ; Xavier Noblin [France]Source :
- Journal of the Royal Society, Interface [ 1742-5662 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Potassium.
- effets des médicaments et des substances chimiques : Phytophthora, Spores fongiques.
- pharmacologie : Potassium.
- physiologie : Phytophthora, Spores fongiques.
- Chimiotaxie.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Potassium.
- drug effects : Phytophthora, Spores, Fungal.
- chemical , pharmacology : Potassium.
- physiology : Phytophthora, Spores, Fungal.
- Chemotaxis.
Abstract
The biflagellate zoospores of some phytopathogenic Phytophthora species spontaneously aggregate within minutes in suspension. We show here that Phytophthora parasitica zoospores can form aggregates in response to a K+ gradient with a particular geometric arrangement. Using time-lapse live imaging in macro- and microfluidic devices, we defined (i) spatio-temporal and concentration-scale changes in the gradient, correlated with (ii) the cell distribution and (iii) the metrics of zoospore motion (velocity, trajectory). In droplets, we found that K+-induced aggregates resulted from a single biphasic temporal sequence involving negative chemotaxis followed by bioconvection over a K+ gradient concentration scale [0-17 mM]. Each K+-sensing cell moved into a region in which potassium concentration is below the threshold range of 1-4 mM, resulting in swarming. Once a critical population density had been achieved, the zoospores formed a plume that migrated downward, with fluid advection in its wake and aggregate formation on the support surface. In the microfluidic device, the density of zoospores escaping potassium was similar to that achieved in droplets. We discuss possible sources of K+ gradients in the natural environment (zoospore population, microbiota, plant roots, soil particles), and implications for the events preceding inoculum formation on host plants.
DOI: 10.1098/rsif.2019.0367
PubMed: 31387479
PubMed Central: PMC6731506
Affiliations:
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wicri:level="1"><nlm:affiliation>Université Côte d'Azur, INRA, CNRS, ISA, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Côte d'Azur, INRA, CNRS, ISA, Sophia Antipolis</wicri:regionArea><wicri:noRegion>Sophia Antipolis</wicri:noRegion><wicri:noRegion>Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Cohen, Celine" sort="Cohen, Celine" uniqKey="Cohen C" first="Celine" last="Cohen">Celine Cohen</name><affiliation wicri:level="3"><nlm:affiliation>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Nice</settlement></placeName></affiliation></author><author><name sortKey="Thomen, Philippe" sort="Thomen, Philippe" uniqKey="Thomen P" first="Philippe" last="Thomen">Philippe Thomen</name><affiliation wicri:level="3"><nlm:affiliation>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Nice</settlement></placeName></affiliation></author><author><name sortKey="Etienne, Catherine" sort="Etienne, Catherine" uniqKey="Etienne C" first="Catherine" last="Etienne">Catherine Etienne</name><affiliation wicri:level="1"><nlm:affiliation>Université Côte d'Azur, INRA, CNRS, ISA, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Côte d'Azur, INRA, CNRS, ISA, Sophia 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type="abstract" xml:lang="en">The biflagellate zoospores of some phytopathogenic Phytophthora species spontaneously aggregate within minutes in suspension. We show here that Phytophthora parasitica zoospores can form aggregates in response to a K<sup>+</sup> gradient with a particular geometric arrangement. Using time-lapse live imaging in macro- and microfluidic devices, we defined (i) spatio-temporal and concentration-scale changes in the gradient, correlated with (ii) the cell distribution and (iii) the metrics of zoospore motion (velocity, trajectory). In droplets, we found that K<sup>+</sup>-induced aggregates resulted from a single biphasic temporal sequence involving negative chemotaxis followed by bioconvection over a K<sup>+</sup> gradient concentration scale [0-17 mM]. Each K<sup>+</sup>-sensing cell moved into a region in which potassium concentration is below the threshold range of 1-4 mM, resulting in swarming. Once a critical population density had been achieved, the zoospores formed a plume that migrated downward, with fluid advection in its wake and aggregate formation on the support surface. In the microfluidic device, the density of zoospores escaping potassium was similar to that achieved in droplets. We discuss possible sources of K<sup>+</sup> gradients in the natural environment (zoospore population, microbiota, plant roots, soil particles), and implications for the events preceding inoculum formation on host plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31387479</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1742-5662</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>157</Issue><PubDate><Year>2019</Year><Month>08</Month><Day>30</Day></PubDate></JournalIssue><Title>Journal of the Royal Society, Interface</Title><ISOAbbreviation>J R Soc Interface</ISOAbbreviation></Journal><ArticleTitle>Guidance of zoospores by potassium gradient sensing mediates aggregation.</ArticleTitle><Pagination><MedlinePgn>20190367</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1098/rsif.2019.0367</ELocationID><Abstract><AbstractText>The biflagellate zoospores of some phytopathogenic Phytophthora species spontaneously aggregate within minutes in suspension. We show here that Phytophthora parasitica zoospores can form aggregates in response to a K<sup>+</sup> gradient with a particular geometric arrangement. Using time-lapse live imaging in macro- and microfluidic devices, we defined (i) spatio-temporal and concentration-scale changes in the gradient, correlated with (ii) the cell distribution and (iii) the metrics of zoospore motion (velocity, trajectory). In droplets, we found that K<sup>+</sup>-induced aggregates resulted from a single biphasic temporal sequence involving negative chemotaxis followed by bioconvection over a K<sup>+</sup> gradient concentration scale [0-17 mM]. Each K<sup>+</sup>-sensing cell moved into a region in which potassium concentration is below the threshold range of 1-4 mM, resulting in swarming. Once a critical population density had been achieved, the zoospores formed a plume that migrated downward, with fluid advection in its wake and aggregate formation on the support surface. In the microfluidic device, the density of zoospores escaping potassium was similar to that achieved in droplets. We discuss possible sources of K<sup>+</sup> gradients in the natural environment (zoospore population, microbiota, plant roots, soil particles), and implications for the events preceding inoculum formation on host plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Galiana</LastName><ForeName>Eric</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Université Côte d'Azur, INRA, CNRS, ISA, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cohen</LastName><ForeName>Celine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomen</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Etienne</LastName><ForeName>Catherine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Université Côte d'Azur, INRA, CNRS, ISA, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noblin</LastName><ForeName>Xavier</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Université Côte d'Azur, CNRS, UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108 Nice, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>08</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J R Soc Interface</MedlineTA><NlmUniqueID>101217269</NlmUniqueID><ISSNLinking>1742-5662</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>RWP5GA015D</RegistryNumber><NameOfSubstance UI="D011188">Potassium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002633" MajorTopicYN="Y">Chemotaxis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011188" MajorTopicYN="N">Potassium</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Phytophthora</Keyword><Keyword MajorTopicYN="Y">aggregation</Keyword><Keyword MajorTopicYN="Y">bioconvection</Keyword><Keyword MajorTopicYN="Y">negative chemotaxis</Keyword><Keyword MajorTopicYN="Y">zoospore</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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first="Catherine" last="Etienne">Catherine Etienne</name><name sortKey="Noblin, Xavier" sort="Noblin, Xavier" uniqKey="Noblin X" first="Xavier" last="Noblin">Xavier Noblin</name><name sortKey="Thomen, Philippe" sort="Thomen, Philippe" uniqKey="Thomen P" first="Philippe" last="Thomen">Philippe Thomen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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